Transcriptional regulation of inflammation and cancer-related genes
Deng Wuguo's Laboratory

The Deng Laboratory, under the supervision of Dr. Wuguo Deng, studies the transcriptional regulation of inflammation and cancer-related genes and the molecular mechanisms of these genes in regulating tumor development and progression using multidisciplinary approaches and tools including streptavidin bead-based DNA binding protein pull down system, mass spectrometry-based proteomics, siRNA library screening, protein expression and structural analyses, tissue micro array, mammalian cell culture and animal models. The Deng Laboratory is part of the State Key Laboratory of Oncology in South China and the Department of Experimental Research at Sun Yat-sen University Cancer Center.

Current Research
1. Transcriptional regulation of inflammation and cancer-related genes such as COX-2, iNOS,p300/CBP, hTERT.

2. Discovery and identification of the novel cancer biomarkers and therapeutic targets and the mechanisms of actions of candidate genes in regulating tumor cell growth.

Contact Information
Wuguo Deng, Ph.D.
Principle Investigator
Department of Experimental Research
Sun Yat-sen University Cancer Center

651 Dongfeng East Road
Building 2, Room 705
Guangzhou 510060,China


1.       Yu Z, Guo W, Ma X, Zhang B, Dong P,Huang L, Wang X, Wang C, Huo X, Yu W, Yi C, Xiao Y, Yang W, Qin Y, Yuan Y, MengS, Liu Q, Deng W*. Gamabufotalin,a bufadienolide compound from toad venom, suppresses COX-2 expression throughtargeting IKKbeta/NF-kappaB signaling pathway in lung cancer cells. Mol Cancer. 2014 Aug 31;13(1):203. [Epub ahead of print]

2.       Guo W, Lu J, Dai M, Wu T, Yu Z, Wang J,Chen W, Shi D, Xiao Y, Yi C, Tang Z, Yu T, Xiao X, Yuan Y, Kang T, Huang W, Deng W*. Transcriptionalcoactivator CBP upregulates hTERT expression and tumor growth and predicts poorprognosis in human lung cancers. Oncotarget. 2014 Aug 30; [In Press]

3.       Chen W, Wang J, Qin Y, Yu W, Guo W, TuanY, Sji D, Fu L, Kang TB, Huang W, DengW*. Ret finger protein-like 3 promotes tumor cell growth by activating telomerase reversetranscriptase expression in non–smallcell lung cancer cells. Oncotarget. 2014 Sep 7; [InPress]

4.       Liang X, Shi D, Yun J, Mao Y, Ouyang P,Su Z, Fu J, Hou J, Deng W*, Xie F. Friendleukemia virus integration 1 expression has prognostic significance innasopharyngeal carcinoma. Transl Oncol. 2014 Aug;7(4):493-502.

5.       Jiang K, Li Y, Zhu Q, Xu J, Wang Y, Deng W, Liu Q, Zhang G, Meng S. Pharmacologicalmodulation of autophagy enhances Newcastle disease virus-mediated oncolysis indrug-resistant lung cancer cells. BMC Cancer. 2014 Jul 30;14:551.

6.       Yi C, Zhang Y, Yu Z, Xiao Y, Wang J, QiuH, Yu W, Tang R, Yuan Y, Guo W, Deng W*. Melatoninenhances the anti-tumor effect of fisetin by inhibiting COX-2/iNOS andNF-κB/p300 signaling pathways. PLoS One. 2014 Jul 7;9(7):e99943.

7.       Hu K, Liao D, Wu W, Han AJ, Shi HJ, WangF, Wang X, Zhong L, Duan T, Wu Y, Cao J, Tang J, Sang Y, Wang L, Lv X, Xu S,Zhang RH, Deng WG, Li SP, Zeng YX,Kang T. Targeting theanaphase-promoting complex/cyclosome (APC/C)- bromodomain containing 7 (BRD7)pathway for human osteosarcoma. Oncotarget. 2014 May 30;5(10):3088-100.

8.       Lin L, Deng W*, Tian Y, Chen W, Wang J, Fu L, Shi D, Zhao M, Luo W. Lasiodininhibits proliferation of human nasopharyngeal carcinoma cells by simultaneousmodulation of the Apaf-1/caspase, AKT/MAPK and COX-2/NF-κB signaling pathways. PLoS One. 2014 May 20;9(5):e97799.

9.       Fu L, Shi K, Wang J, Chen W, Shi D, TianY, Guo W, Yu W, Xiao X, Kang T, Wang S, Huang W, Deng W*. TFAP2B overexpressioncontributes to tumor growth and a poor prognosis of human lung adenocarcinomathrough modulation of ERK and VEGF/PEDF signaling. Mol Cancer. 2014 Apr 26;13:89.

10.   Ye W, Liu R, Pan C, Jiang W, Zhang L,Guan Z, Wu J, Ying X, Li L, Li S, Tan W, Zeng M, Kang T, Liu Q, Thomas GR,Huang M, Deng W, Huang W. Multicenterrandomized phase 2 clinical trial of a recombinant human endostatin adenovirusin patients with advanced head and neck carcinoma. Mol Ther. 2014 Jun;22(6):1221-9.

11.   Chen W, Qin L, Wang S, Li M, Shi D, TianY, Wang J, Fu L, Li Z, Guo W, Yu W, Yuan Y, Kang T, Huang W, Deng W*. CPSF4 activates telomerasereverse transcriptase and predicts poor prognosis in human lungadenocarcinomas. Mol Oncol. 2014May;8(3):704-16.

12.   Shi D, Xie F, Zhang Y, Tian Y, Chen W,Fu L, Wang J, Guo W, Kang T, Huang W, DengW*. TFAP2A regulatesnasopharyngeal carcinoma growth and survival by targeting HIF-1α signalingpathway. Cancer Prev Res (Phila). 2014 Feb;7(2):266-77.

13.   Chen W, Guo W, Li M, Shi D, Tian Y, LiZ, Wang J, Fu L, Xiao X, Liu QQ, Wang S, Huang W, Deng W*. Upregulation of cleavageand polyadenylation specific factor 4 in lung adenocarcinoma and its criticalrole for cancer cell survival and proliferation. PLoS One. 2013 Dec 16;8(12):e82728.

14.   Ouyang PY, Su Z, Mao YP, Liang XX, LiuQ, Deng W*, Xie FY. Prognosticimpact of cigarette smoking on the survival of patients with establishednasopharyngeal carcinoma. CancerEpidemiol Biomarkers Prev. 2013 Dec;22(12):2285-94.

15.   Ouyang PY, Su Z, Mao YP, Deng W*, Xie FY. Combinationof EGFR-TKIs and chemotherapy as first-line therapy for advanced NSCLC: ameta-analysis. PLoS One. 2013Nov 13;8(11):e79000.

16.   Fu L, Chen W, Guo W, Wang J, Tian Y, ShiD, Zhang X, Qiu H, Xiao X, Kang T, Huang W, Wang S, Deng W*. Berberine TargetsAP-2/hTERT, NF-κB/COX-2, HIF-1α/VEGF and Cytochrome-c/Caspase Signaling toSuppress Human Cancer Cell Growth. PLoS One. 2013 Jul 15;8(7):e69240.

17.    Wang L, Yuan C, Lv K, Xie S, Fu P, Liu X, Chen Y, Qin C, Deng W*, Hu W. Lin28mediates radiation resistance of breast cancer cells via regulation of caspase,H2A.X and Let-7 signaling. PLoS One.2013 Jun 20;8(6):e67373.

18.    Wang J, Liu L, Qiu H, Zhang X, Guo W, Chen W, Tian Y, FuL, Shi D, Cheng J, Huang W, Deng W*.Ursolic acid simultaneously targets multiple signaling pathwaysto suppress proliferation and induce apoptosis in colon cancer cells. PLoS One. 2013 May 30;8(5):e63872.

19.    Wang J, Guo W, Chen W, Yu W, Tian Y, Fu L, Shi D, Tong B,Xiao X, Huang W, Deng W*. Melatoninpotentiates the antiproliferative and pro-apoptotic effects of ursolic acid incolon cancer cells by modulating multiple signaling pathways. J Pineal Res. 2013 May;54(4):406-16.

20.   Guo W, Chen W, Yu W, Huang W, Deng W*. Small interferingRNA-based molecular therapy of cancers. Chin J Cancer.2013 Sep;32(9):488-93.

21.    Qin Y, Deng W,Ekmekcioglu S, Grimm EA. Identification of unique sensitizing targets foranti-inflammatory CDDO-Me in metastatic melanoma by a large-scale syntheticlethal RNAi screening. Pigment Cell MelanomaRes. 2013 Jan;26(1):97-112.

22.   Shi D, Guo W, Chen W, Fu L, Wang J, TianY, Xiao X, Kang T, Huang W, Deng W*. Nicotinepromotes proliferation of human nasopharyngeal carcinoma cells by regulatingα7AChR, ERK, HIF-1α and VEGF/PEDF signaling. PLoS One. 2012;7(8):e43898.

23.   Lv K, Liu L, Wang L, Yu J, Liu X, ChengY, Dong M, Teng R, Wu L, Fu P, Deng W*,Hu W, Teng L. Lin28 mediates paclitaxelresistance by modulating p21, Rb and Let-7a miRNA in breast cancer cells. PLoS One. 2012;7(7):e40008.

24.   Shi D, Xiao X, Wang J, Liu L, Chen W, FuL, Xie F, Huang W, Deng W*. Melatoninsuppresses proinflammatory mediators in lipopolysaccharide-stimulated CRL1999cells via targeting MAPK, NF-κB, c/EBPβ, and p300 signaling. J Pineal Res. 2012 Sep;53(2):154-65.

25.   Wang J, Xiao X, Zhang Y, Shi D, Chen W,Fu L, Liu L, Xie F, Kang T, Huang W, DengW*. Simultaneous modulation ofCOX-2, p300, Akt, and Apaf-1 signaling by melatonin to inhibit proliferationand induce apoptosis in breast cancer cells. J Pineal Res. 2012 Aug;53(1):77-90.

26.   Tang QL, Xie XB, Wang J, Chen Q, Han AJ,Zou CY, Yin JQ, Liu DW, Liang Y, Zhao ZQ, Yong BC, Zhang RH, Feng QS, Deng WG, Zhu XF, Zhou BP, Zeng YX, ShenJN, Kang T. Glycogen synthasekinase-3β, NF-κB signaling, and tumorigenesis of human osteosarcoma. J Natl Cancer Inst. 2012 May 16;104(10):749-63.

27.    DengWG,Kwon J, Ekmekcioglu S, Poindexter NJ, Grimm EA. IL-24 gene transfersensitizes melanoma cells to erlotinib through modulation of the Apaf-1 and Aktsignaling pathways. Melanoma Res. 2011 Feb;21(1):44-56.

28.   Xiao X, Shi D, LiuL, Wang J, Xie X, Kang T, Deng W*. Quercetinsuppresses cyclooxygenase-2 expression and angiogenesis through inactivation ofP300 signaling. PLoS One.2011;6(8):e22934.

29.    DengWG,Wu G, Ueda K, Xu K, Roth JA, Ji L. Enhancementof antitumor activity of cisplatin in human lung cancer cells by tumorsuppressor FUS1. Cancer Gene Ther.2008 Jan;15(1):29-39.

30.    DengWG,Jayachandran G, Wu G, Xu K, Roth JA, Ji L. Tumor-specific activationof human telomerase reverses transcriptase promoter activity by activatingenhancer-binding protein-2beta in human lung cancer cells. J Biol Chem. 2007 Sep 7;282(36):26460-70.

31.    DengWG,Montero AJ, Wu KK. Interferon-gammasuppresses cyclooxygenase-2 promoter activity by inhibiting C-Jun and C/EBPbetabinding. Arterioscler Thromb Vasc Biol. 2007 Aug;27(8):1752-9.

32.   Lin J, Sun T, Ji L, Deng W, Roth J, Minna J, Arlinghaus R. Oncogenicactivation of c-Abl in non-small cell lung cancer cells lacking FUS1expression: inhibition of c-Abl by the tumor suppressor gene product Fus1. Oncogene. 2007 Oct 25;26(49):6989-96.

33.   Ohtani S, Iwamaru A, Deng W, Ueda K, Wu G, Jayachandran G,Kondo S, Atkinson EN, Minna JD, Roth JA, Ji L. Tumor suppressor 101F6 andascorbate synergistically and selectively inhibit non-small cell lung cancergrowth by caspase-independent apoptosis and autophagy. Cancer Res. 2007 Jul 1;67(13):6293-303.

34.    DengWG,Nishizaki M, Fang B, Roth JA, Ji L. Inductionof apoptosis by tumor suppressor FHIT via death receptor signaling pathway inhuman lung cancer cells. Biochem BiophysRes Commun. 2007 Apr 20;355(4):993-9.

35.    DengWG, Kawashima H, Wu G, Jayachandran G, Xu K, Minna JD, Roth JA, Ji L. Synergistictumor suppression by coexpression of FUS1 and p53 is associated withdown-regulation of murine double minute-2 and activation of the apoptoticprotease-activating factor 1-dependent apoptotic pathway in human non-smallcell lung cancer cells. Cancer Res. 2007 Jan 15;67(2):709-17.

36.   Ueda K, Kawashima H, Ohtani S, Deng WG, Ravoori M, Bankson J, Gao B,Girard L, Minna JD, Roth JA, Kundra V, Ji L. The 3p21.3 tumorsuppressor NPRL2 plays an important role in cisplatin-induced resistance inhuman non-small-cell lung cancer cells. Cancer Res.2006 Oct 1;66(19):9682-90.

37.   Cieslik KA, Deng WG, Wu KK. Essentialrole of C-Rel in nitric-oxide synthase-2 transcriptional activation:time-dependent control by salicylate. Mol Pharmacol.2006 Dec;70(6):2004-14.

38.    DengWG,Tang ST, Tseng HP, Wu KK. Melatonin suppressesmacrophage cyclooxygenase-2 and inducible nitric oxide synthase expression byinhibiting p52 acetylation and binding. Blood.2006 Jul 15;108(2):518-24.

39.    Deng WG, Zhu Y, Wu KK. Roleof p300 and PCAF in regulating cyclooxygenase-2 promoter activation byinflammatory mediators. Blood.2004 Mar 15;103(6):2135-42.

40.    DengWG, Zhu Y, Montero A, Wu KK. Quantitative analysis ofbinding of transcription factor complex to biotinylated DNA probe by astreptavidin-agarose pulldown assay. Anal Biochem.2003 Dec 1;323(1):12-8.

41.    DengWG,Wu KK. Regulation of induciblenitric oxide synthase expression by p300 and p50 acetylation. J Immunol. 2003 Dec 15;171(12):6581-8.

42.    Deng WG, Zhu Y, Wu KK. Up-regulationof p300 binding and p50 acetylation in tumor necrosis factor-alpha-inducedcyclooxygenase-2 promoter activation. J Biol Chem.2003 Feb 14;278(7):4770-7.

43.    DengWG,Saunders M, Gilroy D, He XZ, Yeh H, Zhu Y, Shtivelband MI, Ruan KH, Wu KK. Purificationand characterization of a cyclooxygenase-2 and angiogenesis suppressing factorproduced by human fibroblasts. FASEB J. 2002 Aug;16(10):1286-8.

44.   Schroer K, Zhu Y, Saunders MA, Deng WG, Xu XM, Meyer-Kirchrath J, WuKK. Obligatory role of cyclicadenosine monophosphate response element in cyclooxygenase-2 promoter inductionand feedback regulation by inflammatory mediators. Circulation.2002 Jun 11;105(23):2760-5.

45.   Zhu Y, Saunders MA,Yeh H, Deng WG, Wu KK. Dynamicregulation of cyclooxygenase-2 promoter activity by isoforms ofCCAAT/enhancer-binding proteins. J BiolChem. 2002 Mar 1;277(9):6923-8.

46.    Deng WG, Ruan KH, Du M,Saunders MA, Wu KK. Aspirinand salicylate bind to immunoglobulin heavy chain binding protein (BiP) andinhibit its ATPase activity in human fibroblasts. FASEB J. 2001 Nov;15(13):2463-70.

47.   Liou JY, Deng WG, Gilroy DW, Shyue SK, Wu KK. Colocalization andinteraction of cyclooxygenase-2 with caveolin-1 in human fibroblasts. J Biol Chem. 2001 Sep 14;276(37):34975-82.

48.    Deng W, HeY, Fang X, Wu J. Radiolysisof rutin in aerated ethanolic solution.Radiat Phys Chem. 1998; 53:629-33.

49.    Deng W, Fang X, Wu J. Flavonoidsfunction as antioxidants: By scavenging reactive oxygen species or by chelatingiron? Radiat Phys Chem. 1997; 50:273-5.

50.   Yi QM, Deng WG, Xia ZP, Pang HH. Polymorphismand genetic relatedness among wild and cultivated rice species determined byAP-PCR analysis. Hereditas.1995;122(2):13ww5-41.

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